LC-MS/MS profiling, antibiofilm, antimicrobial and bacterial growth kinetic studies of Pluchea dioscoridis extracts

Abstract The therapeutical applications of ornamental plants have been categorized to be a great effectiveness in multiple industries from ancient times until present days. Pluchea dioscoridis is widely known Egyptian wooden plant that has extensively applied for different medicinal purposes. In this study, LC-ESI-MS/MS analysis the potent antimicrobial ethyl acetate and n -butanol extracts P. leaves led identification 28 21 compounds, respectively. identified compounds were as phenolic acids, acids derivatives, organic flavonoids (aglycones glycosides), secoiridoids, coumarin gallotannins derivatives. Among them, caffeic acid 3-sulfate was most predominate investigated followed by ferulic dicaffeoyl-quinic acid. Also, potentiality evaluated against pathogenic microbes including Enterobacter cloacae , Micrococcus leutus Aeromonas hydrophila Bacillus subtilis cereus lichneformis Clostridium sp ecies. Furthermore, concentrations extract assayed antibacterial efficacy on growth curve kinetics susceptible bacteria along 4days incubation period. Our gathered data confirmed that, activity tested according solvent used extraction process. Mostly, all showed wide spectrum except water which shows mild sp. only. Based highest inhibition zone diameter, butanol exhibited with luteus B. (20.0 18.5 mm) Determining effect at concentration (0, 0.66, 1.66, 3.33, 6.67, 13.34 20.01 mg mL −1 ) M. kinetics, assured dependent manner culture. also none selected bactericidal prepared cultures, prolonged period acquire resistance beginning second or third day incubation.